Technologies

Core technologies that already have been researched for our goal

• CRISPR-Cas9
  • It is a powerful tool for gene editing. It is adapted from the natural defense mechanisms of bacteria. When DNA is cut by CRISPR, DNA starts to repair itself.

• Adipose-derived Mesenchymal Stem Cell (AdMSC) Differentiation
  • Isolated mesenchymal stem cells from adipose can differentiate into myocyte, adipocyte, Osteoblast, Neuron, or Chondrocyte. This differentiation leads us to use AdMSC for LGMD2B research.

• Muscle Stem Cell (MuSC)
  • Muscle stem cells are present in skeletal muscle tissue and are able to self-regeneration when muscle is damaged.

• 3D Stem Cell Culture
  • Unlike 2D stem cell culturing in petri dish, 3D stem cell culturing allows researchers to have more advanced experiments with better cell environments.

• Skeletal Muscle Organoids
  • It is a self-organized tissue that are derived from stem cells. It replicates an organ or certain type of cells.

• Muscle Engraftment
  • The most important stage for LGMD2B disease treatment. Edited gene must be engrafted on the target muscle site after infusion in the body.